A fully automated novel solid-phase extraction method for siRNA drug extraction and quantitation across multiple tissues using LC-MS
This manuscript presents a one-step SPE method greatly reducing blank tissue requirements and sample preparation workload by enabling use of surrogate matrix calibration and full automation. The presented SPE method simplifies and reduces analytical time in bioanalysis of tissue biodistribution studies and adheres to the principles of 3R.
Authors
- Mikko Hölttä (ORCID: https://orcid.org/0000-0001-6024-3686)
- Carl L. Stenbratt (ORCID: https://orcid.org/0000-0003-1049-6532)
- Amalyn Nain-Perez
- Tony Karlsborn
Institutions
- AstraZeneca (Brazil) (BR)
Publication Details
- Journal
- Bioanalysis
- Published
- 2026-01-22
- DOI
- https://doi.org/10.1080/17576180.2026.2636553
- Primary Topic
- Advanced Proteomics Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00