Construction and application of multiple nucleotide polymorphism-based DNA fingerprinting for Polygonatum cyrtonema identification

The MNP marker system developed in this study combines the advantages of high throughput, high accuracy, and favorable reproducibility. It provides the technical means and solutions for the authenticity identification of <i>P. cyrtonema</i> cultivars, purity detection of seeds and seedlings, conservation of germplasm resources, as well as the conduct of distinctness, uniformity, and stability testing for new plant varieties and the application for new variety protection rights. These findings are expected to be applied for the source authentication of medicinal plants and population genetics research.