Simultaneous Determination of Glucose and Cholesterol in Milk Samples by Means of a Screen-Printed Biosensor and Artificial Neural Networks

In the present work, a bienzymatic voltammetric biosensor is reported for the simultaneous quantification of glucose and cholesterol in flavored milk samples with a single device. The biosensor is based on a carbon screen printing electrode on which paper disks impregnated with glucose oxidase and cholesterol oxidase, as well as ferricyanide as a mediator, are deposited. Linear voltammetry combined with an artificial neural network methodology were successfully used for the determinations, showing excellent agreement with the spectrophotometric reference method.