Zeolite-gold nanoparticle composite mediated gynecological cancer identification on interdigitated electrode

Gynecological cancer refers to that originating in female reproductive organs, such as the uterus, ovaries, cervix, vagina, and vulva. Early diagnosis helps to provide ideal treatment options and better outcomes. Human papillomavirus (HPV) infection is a major cause of different types of gynecological cancer, in particular ovarian and cervical cancers. This research was focused on developing a highly sensitive HPV biosensor on a nanomaterial-mediated interdigitated electrode sensor. Zeolite nanomaterial was modified on the electrode, and then gold nanoparticle (AuNP)-conjugated capture DNA was immobilized on the zeolite. AuNPs improve the captured DNA immobilization on sensing surfaces, and then a sandwich assay of capture-target-reporter DNA was conducted for Human papilloma virus-16 DNA detection. Further, to enhance the sensing, reporter DNA was attached to AuNPs for the sandwich assay and lowered the target DNA limit of detection to 1 aM on a linear regression [y = 1.3326x - 2.2747; R 2 = 0.9196]. In addition, control DNAs did not show notable current changes, confirming specific target DNA identification. This HPV-16 DNA biosensor identifies the virus infection earlier and diagnoses gynecological cancer and its related issues.