Preparation of Bacterial Biofilm Colonies for Morphological Analysis

Source: Bucher, T., et. al., Methodologies for Studying B. subtilis Biofilms as a Model for Characterizing Small Molecule Biofilm Inhibitors. J. Vis. Exp.(2016)This video demonstrates the preparation of mature bacterial biofilm colonies for high-resolution imaging. It covers fixation, dehydration, mounting, and metal coating steps to preserve and visualize biofilm morphology.

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Publication Details

Journal
Journal of Visualized Experiments
Published
2026-02-26
DOI
https://doi.org/10.3791/24490
Primary Topic
Bacterial biofilms and quorum sensing
Type
article
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article

Preparation of Bacterial Biofilm Colonies for Morphological Analysis

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Journal of Visualized Experiments
Bacterial biofilms and quorum sensing
article

Preparation of Bacterial Biofilm Colonies for Morphological Analysis

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Abstract

Source: Bucher, T., et. al., Methodologies for Studying B. subtilis Biofilms as a Model for Characterizing Small Molecule Biofilm Inhibitors. J. Vis. Exp.(2016)This video demonstrates the preparation of mature bacterial biofilm colonies for high-resolution imaging. It covers fixation, dehydration, mounting, and metal coating steps to preserve and visualize biofilm morphology.

Journal of Visualized Experiments
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Openalex Percentile: Top 41%
Bacterial biofilms and quorum sensing
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