Protocol for the generation of a human-derived nasal epithelial model and induction of tissue-resident memory-like T cells in a co-culture system
Tissue-resident memory T cells (TRMs) are a distinct subset of T lymphocytes that permanently localize in non-lymphoid tissues and mediate rapid, localized immune responses. Here, we present a protocol to study human TRMs using an in vitro model system that induces TRM-like T cells. We describe steps for generating the 3D nasal epithelial model and inducing TRMs via co-culture with autologous immune cells. We then detail procedures to analyze TRMs using flow cytometry, single-cell RNA sequencing, and cytokine release.
Authors
- Youvika Singh (ORCID: https://orcid.org/0000-0002-4274-4484)
- Anne M. van der Does (ORCID: https://orcid.org/0000-0002-1613-106X)
- Wesley Huisman (ORCID: https://orcid.org/0000-0002-5699-7087)
- Simon P. Jochems (ORCID: https://orcid.org/0000-0002-4835-1032)
- Geert H. Groeneveld (ORCID: https://orcid.org/0000-0001-6529-1396)
- Lisa A. King (ORCID: https://orcid.org/0009-0001-8705-150X)
- Iris van der Valk
- Pieter S. Hiemstra
- Jasmijn S. Schrumpf
Institutions
- Leiden University Medical Center (NL)
Publication Details
- Journal
- STAR Protocols
- Published
- 2026-02-26
- DOI
- https://doi.org/10.1016/j.xpro.2026.104401
- Primary Topic
- T-cell and B-cell Immunology
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- European Federation of Pharmaceutical Industries and Associations
- European Commission
- Universiteit Leiden
- Nederlandse Organisatie voor Wetenschappelijk Onderzoek
- Leids Universitair Medisch Centrum