Effect of early to mid-gestation heat stress exposure on mammary development and milk traits in F1 gilts divergently selected for thermotolerance

Heat stress (HS) reduces milk yield in sows independent of lactation feed intake, leading to poorer piglet growth and survivability. Therefore, the study objectives were to evaluate the impacts of early to mid-gestation HS versus thermoneutral (TN) conditions on biomarkers of mammary development during late gestation and milk quality during lactation, and to determine whether genomic selection for HS tolerance (TOL) or HS sensitivity (SEN), would influence these measures. We hypothesized that exposure to HS during early to mid-gestation would adversely affect biomarkers of mammary development in late gestation and milk quality during lactation, and that these adverse effects would be attenuated in individuals with higher genetic merit for TOL compared with SEN. Gilts, divergently selected for TOL (n = 15) or SEN (n = 13), were balanced by weight, bred to TOL or SEN boars, respectively and then exposed to either TN (21.46 ± 2.92 °C; n = 7 SEN and n =8 TOL) or cyclical HS conditions (26 to 36 °C; n = 6 SEN and n =7 TOL) from gestation d 6.3 ± 0.9 to 65.3 ± 0.9. All animals were then maintained under TN conditions (21.40 ± 3.13 °C) until farrowing. On gestation d 105.3 ± 0.9, a mammary biopsy was collected from all gilts, fixed in 10% buffered formalin, and stained using Ki67 immunohistochemistry and hematoxylin and eosin to identify proliferating mammary epithelial cells (MEC) and estimate alveolar and lumen sizes. Both proliferating and non-proliferating MEC counts, as well as lobuloalveolar size estimations, were performed using ImageJ software. Milk samples collected on d 0, 3, 10, and 14 of lactation were assessed for milk components. Data were analyzed using PROC GLIMMIX in the SAS 9.4 software, with individual gilt as the experimental unit. Regardless of the genetic line, HS exposure in early to mid-gestation reduced (P < 0.01) late gestation MEC proliferation by 37.5% and increased (P = 0.02) mammary epithelial lumen to alveolar ratio (L:A) by 15.0%. Within the TOL line, L:A was greater in TOL+HS gilts (P < 0.01; 28.2%) when compared to TOL+TN gilts. Milk sodium to potassium ratio, a marker of tight junction integrity, was reduced (P = 0.04) in TOL (0.30 ± 0.06) versus SEN gilts (0.44 ± 0.06), regardless of gestation environment. In conclusion, early to mid-gestation HS negatively impacted biomarkers of mammary development, and genomic selection for TOL improved some measures of MEC integrity relative to SEN F1 replacement gilts.