A Rapid Image-Based Assay for Screening Surface-Induced Bacterial Virulence

Source: Perinbam, K., and Siryaporn, A. A Rapid Image-based Bacterial Virulence Assay Using Amoeba. J. Vis. Exp. (2018)This video demonstrates a rapid, image-based method to assess bacterial virulence by incubating amoebae with surface-attached or planktonic bacteria, applying a calcein-acetoxymethyl ester (calcein-AM) agarose pad, and imaging fluorescence to detect host cell damage.