Extraction and Isolation of Active Fraction from

Ganoderma boninense is a fungus that causes basal stem rot disease in oil palm plants. This fungus disrupts the transport of water and nutrients from the soil, leaf chlorosis, stem decay, fruitlessness, and ultimately death. This study is one of the first to specifically explore the bark of Senna multijuga as a source of antifungal compounds against Ganoderma boninense. Previous studies have focused more on leaf extracts from other Senna species, so the use of S. multijuga bark provides a new contribution to the search for biological agents to control G. boninense. This study aims to extract and isolate the active fraction from S. multijuga bark as antifungal agents against G. boninense. The study was conducted in three stages. The extraction and isolation of the active fraction from S. multijuga bark as antifungal agents against G. boninense, the determination of the chemical composition of the S. multijuga bark, and the antifungal activity of the active fraction against G. boninense in vitro. Based on the GC-MS results for fraction-1 from S. multijuga bark showed the main compounds of 1-hexadecene (5.51%), 1-octadecene (8.29%), hexadecanoid acid (10.46%), 2-ethoxycyclohexanol (5.53%), 9,12-octadecadienoic acid (8.91%), 9,12,15-octadecatrienoic acid (7.70%), 2-hexadecen-1-ol (5.48%), 1-docosene (3.33%), 2-pyridol (6.28%), and calotropenol acetate (6.52%). A concentration of fraction-1 at 1.50 ppm can inhibit the growth of G. boninense (0.32 cm/day), inhibition of G. boninense (67,46%), and produce thin white mycelium that collects in the middle of the medium.