Cloning and expression analysis of the HvLRR-RLK-510 gene encoding a leucine-rich repeat receptor-like kinase in barley

Elucidating the function and regulatory mechanisms of LRR-RLK (leucine-rich repeat receptor-like kinase) genes is essential for understanding the developmental processes and stress responses in barley. In this study, eight barley varieties or lines, including Beiqing 7, the Ynbs mutant, and Morex, were used as experimental materials. A 2904 bp coding sequence (CDS) of the <italic>HvLRR-RLK-510</italic> gene was successfully cloned. The CDS and corresponding amino acid sequences of <italic>HvLRR-RLK-510</italic> all showed over 99.00% similarity, across the eight genotypes. The encoded protein contains key conserved domains typical of LRR-RLKs, including leucine-rich repeat domains, transmembrane regions, and a kinase domain. Phylogenetic analysis grouped this protein into a subclass shared with LRR-RLKs from wheat, rice, and <italic>Arabidopsis</italic>. RT-PCR and quantitative real-time PCR analyses revealed that <italic>HvLRR-RLK-510</italic> was differentially expressed in young spikes, leaf sheaths, leaves, roots, and stems of the above-mentioned eight materials at the jointing stage, exhibiting both organ specificity and genotype dependence. Notably, expression in young spikes was consistently higher and less affected by genotype. Subcellular localization experiments showed that the protein is localized to the plasma membrane and vacuolar membrane in <italic>Nicotiana benthamiana</italic> leaf cells. This study provides a valuable gene resource and theoretical foundation for further investigation into the functional roles and molecular mechanisms of <italic>HvLRR-RLK-510</italic> in barley.