Effect of Undaria pinnatifida extract on wound healing and pro-inflammatory and anti-inflammatory cytokine modulation

Purpose: The aim of this study was to investigate the effects of U. pinnatifida extract on the pro-inflammatory and anti-inflammatory cytokine profile during wound healing at 24, 48, and 72 hours in a scratch wound healing assay performed using the CCD-1072Sk human skin fibroblast cell line. Materials and Methods: CCD-1072Sk Fibroblast cell line was cultured, and U. pinnatifida was extracted. A viability test was performed, and 0.24 µl/ml and 0.5 µl/ml doses of U. pinnatifida were selected. Scratch Wound Healing Assay was performed. Samples were collected at 0, 24, 48, and 72 hours. Interleukin-1 beta (IL-1β), tumor necrosis factor-alpha (TNF-α), interleukin-10 (IL-10), transforming growth factor-beta 1 (TGF-β1), and brain-derived neurotrophic factor (BDNF) levels were determined by ELISA, and apoptosis was determined by Annexin-V-FITC/PI staining. Proliferation and cell migration were examined. Results: In the viability test, 0.24 µl/ml (115.2±2.8) and 0.5 µl/ml (126.7±8.2) of U. pinnatifida were found to be significant at 72 hours compared to the control group (CTL) (100.0±4.1). The most intense and fastest wound closure was observed in cells treated with U-0.5 μL at 72 hours. BDNF and anti-inflammatory IL-10 increased compared to CTL at 72 hours in 0.5 µl/ml. TNF-α and IL-1β decreased in all time points compared to CTL. Conclusion: U. pinnatifida extract, applied at low and high doses, can significantly support wound healing and play a role in precisely controlling the healing process.