UHPLC–MS/MS Quantification of Human Cytosolic Aldehyde Oxidase: Heat‐Assisted In‐Solution Protein Denaturation and Digestion
Our novel methodological advances, as highlighted by a high temperature, in-solution protein denaturation protocol and optimal digestion time, solvent composition, and matrix blank, enabled the femtomole quantification of AOX1 protein using a validated UHPLC-MS/MS method with better assay performance than previous methods.
Authors
- Aik Jiang Lau
Institutions
- Dalhousie University (CA)
- National University of Singapore (SG)
Publication Details
- Journal
- Rapid Communications in Mass Spectrometry
- Published
- 2026-02-25
- DOI
- https://doi.org/10.1002/rcm.70058
- Primary Topic
- Alcohol Consumption and Health Effects
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Natural Sciences and Engineering Research Council of Canada
- Medical Research Council
- National Medical Research Council