Reversing T cell dysfunction in a novel in vitro model of T cell exhaustion reveals differential roles of RASA2
This work provides the first demonstration of CRISPR editing directly in <i>in vitro</i> generated human exhausted T cells, revealing distinct roles for RASA2 across CD4<sup>+</sup> and CD8<sup>+</sup> compartments. This platform enables mechanistic dissection of T cell exhaustion biology with increased throughput and physiological relevance, ultimately supporting the development of novel strategies to overcome cancer immunotherapy resistance.
Authors
- Dympna J. Connolly
- Lindsay Lim (ORCID: https://orcid.org/0000-0003-1394-3297)
- Meera Augustus
- Raha Taghavi
- Sofia Lourenco
- Hilal Saraç
- Rachael Nicholson
- Rebecca N. Graham
Institutions
- Horizon Discovery Group (United States) (US)
Publication Details
- Journal
- Frontiers in Immunology
- Published
- 2026-02-25
- DOI
- https://doi.org/10.3389/fimmu.2026.1509926
- Primary Topic
- Cancer Immunotherapy and Biomarkers
- Type
- article
- Field-Weighted Citation Impact
- 0.00