Effects of Seed Pre-Treatments on Moringa oleifera (Lam.) Germination: Advancing Sustainable Cultivation of a Multipurpose Plant Species

Moringa oleifera (Lam.) is a multipurpose agroforestry tree cultivated worldwide for its nutritional, medicinal, and economic value, and it is increasingly grown commercially in subtropical regions, including Nepal. While vegetative propagation is feasible, large-scale production relies predominantly on seeds, making efficient germination critical for seedling establishment, uniform growth, sustainable production, and preservation of genetic diversity. Seed pre-treatments are widely recognized as a simple and effective approach to enhance germination, early seedling vigor, and nursery performance. This study evaluated the effects of seven pre-sowing treatments under controlled nursery conditions to determine the most effective method for improving Moringa oleifera seedling production. A total of 2100 seeds were used, with 100 seeds per treatment and three replicates, arranged in a Completely Randomized Design (CRD). Treatments included control (no pretreatment), normal water soaking (12 h and 24 h), alternating wetting (water) and drying cycles (12 h each), hot water soaking (60 °C for 5 min), cow urine soaking (1:2 of urine to water proportions for 12 h), and hydrochloric acid soaking (35% for 20 min). All pre-treatments were conducted at room temperature, and the seeds were subsequently sown in controlled nursery conditions. Seed germination was monitored twice daily for 30 days, and data were analyzed using one-way ANOVA and Tukey’s HSD test to identify significant differences in germination performances. Results demonstrated that alternating wetting and drying produced the highest germination percentage (89%), shortest mean germination time (8.44 days), and strongest seedling vigor, outperforming all other treatments. Conversely, cow urine and acid treatments completely inhibited germination. This study recommends alternating wetting and drying as a simple, low-cost, and chemical-free pre-treatment to optimize Moringa oleifera seedling production in nurseries. These findings provide practical guidance for commercial and smallholder farmers, contributing to sustainable agroforestry, food security, and climate-resilient livelihoods in resource-limited habitats.