Narrative Review of <i>Chlamydia trachomatis</i> Culture, Viability Testing, and Sequencing Methods

The need for CT culture in routine clinical practice is declining, but culture continues to play a role in research, surveillance, and select clinical scenarios. Significant gaps in current technology include the inability to distinguish viable from nonviable organisms and lack of standardized AST protocols and genotypic resistance assays. Future research should prioritize development of clinical viability assays, consensus AST methods, and integrated genomic approaches to improve diagnostics and strengthen surveillance.