Phenotypic Antimicrobial Resistance Profiles and Provisional Epidemiological Cut-Off Values of Edwardsiella anguillarum Isolated from Farmed Nile Tilapia (Oreochromis niloticus) in Brazil, with Exploratory Data on Edwardsiella tarda

Antimicrobial resistance in bacteria associated with aquaculture, such as <i>Edwardsiella</i> spp., represents an emerging challenge because of their relevance to fish health and their potential impact on animal, environmental, and human health. In this study, we primarily investigated the antimicrobial susceptibility profiles of <i>Edwardsiella anguillarum</i> isolated from farmed Nile tilapia (<i>Oreochromis niloticus</i>) in Brazil. Based on our findings, herein, we propose provisional local epidemiological cut-off values (pECVs) using the normalized resistance interpretation method, with data for <i>Edwardsiella tarda</i> included as an exploratory context. Fifty isolates (31 <i>E. anguillarum</i> and 19 <i>E. tarda</i>) collected between 2017 and 2025 were tested against 28 antibacterial agents using the disk diffusion method. Based on the pECVs, isolates were classified as wild type (WT) or non-WT (NWT), and the multiple antibiotic resistance (MAR) index was calculated. Most <i>E. anguillarum</i> isolates remained susceptible to several classes, although NWT and multidrug-resistant profiles were detected with a MAR index of 0.68, suggesting selective pressure in intensive tilapia farming systems. These findings support the use of local, species-specific pECVs for resistance surveillance in aquaculture, highlighting the importance of continuous antimicrobial resistance monitoring in aquaculture from a One Health perspective.