Higher sensitivity to ouabain‐induced toxicity in human induced pluripotent stem cell‐derived cardiomyocytes than human adult heart tissue despite similar Na <sup>+</sup> /K <sup>+</sup> ‐ATPase pump current amplitudes
HiPSC-EHTs express NKA with biophysical characteristics and ouabain-sensitivity that are not different from human tissue. Action potential duration responses in EHT reproduces chamber-specific response pattern as seen in human heart. The higher sensitivity of EHTs to depolarize under ouabain-induced block of NKA needs to be considered when employing hiPSC-CM in drug research.
Authors
- Torsten Christ (ORCID: https://orcid.org/0000-0001-5925-4070)
- Umber Saleem (ORCID: https://orcid.org/0000-0002-0466-3851)
- Cristina E. Molina (ORCID: https://orcid.org/0000-0003-3094-1568)
- Margaret Nandudu
- Muhammed Sönmez (ORCID: https://orcid.org/0009-0004-6040-3757)
- Djemail Ismaili (ORCID: https://orcid.org/0000-0001-8709-0448)
- Johannes Petersen
- Thomas Eschenhagen
- Paavo Virtanen
- Paul Brunnbauer
- Joris Winters
- Uli Schotten
- A Hansen
- Carl Schulz
- Junsoo Im
- Jussi T Koivumäki
Institutions
- Universität Hamburg (DE)
- Tampere University (FI)
- Maastricht University (NL)
- University Medical Center Hamburg-Eppendorf (DE)
- Institute of Human Genetics (PL)
- German Centre for Cardiovascular Research (DE)
- Ohio Heart & Vascular Center (US)
Publication Details
- Journal
- British Journal of Pharmacology
- Published
- 2026-02-23
- DOI
- https://doi.org/10.1111/bph.70380
- Primary Topic
- Cardiac electrophysiology and arrhythmias
- Type
- article
- Field-Weighted Citation Impact
- 0.00