Stephania japonica: A Pharmacognostic Study for the in-vivo Wound Healing Activity using Herbal Ointment Formulated from Ethanolic Extract

Stephania japonica plants appear to be rich in secondary metabolites, widely utilised in traditional medicine to treat and heal numerous illnesses. It is traditionally used to treat wounds, but there is no scientific data on its in vivo wound-healing activity. Pharmacognostical analysis evaluates a drug's identity, quality, purity, and safety through microscopic and macroscopic research. The extraction solvent dissolves significant amounts of the target material. The percentage yields of alcohol and water-soluble extractive values were calculated. After the extraction of the plant material, the herbal ointment was formulated. After checking the evaluation parameter of the ointment, the excision wound healing activity was performed on Wistar rats. The herbal extract was prepared by using a simple Soxhlet extraction process to obtain a good yield of extract, and there was no harm to the chemical constituents and their activity. This study showed that the herbal ointment heals wounds better in less time as similar with the standard ointment by applied to Wistar rats. Keywords: Pharmacognostical, Phytochemical, Wound healing, <i>Stephania japonica</i>, Herbal ointment