Chromosome-scale genome assembly and annotation of Garuga floribunda var. gamblei (King ex W. W. Sm.) Kalkman

Garuga floribunda var. gamblei (King ex W. W. Sm.) Kalkman, a tree species of Burseraceae (Sapindales), is rare in China and prized for its high-quality timber. Despite its economic and ecological significance, genetic architecture remains poorly understood, underscoring the necessity for comprehensive genomic studies. In this study, we present a high-quality, chromosome-level genome assembly of G. floribunda var. gamblei, constructed using a combination of PacBio HiFi long reads, Illumina short reads, and Hi-C sequencing technologies. The assembled genome spans approximately 448.57 Mb with a scaffold N50 of 30.54 Mb, and 13 pseudochromosomes were successfully constructed. BUSCO analysis indicated a high level of completeness, with 97.7% of conserved genes detected. Repeat annotation identified 160.78 Mb of repetitive sequences, accounting for 35.84% of the genome. The genome annotation predicted 19,620 protein-coding genes and 14,552 non-coding RNAs. This high-quality genomic resource lays a solid foundation for future comparative genomic studies and provides valuable insights into the evolutionary history and genetic diversity of Burseraceae and related lineages.