Geometrically Well‐Controlled Wireframe RNA Nanostructures With Bundled‐Helix Edges

Wireframe RNA nanostructures with defined geometry are desirable, but bendable RNA edges can lead to structural deformation. Here, we present our design of single-stranded RNA Origami with bundled duplexes as edges. With dual duplex bundles as the wireframe edges, a specific geometry results for our RNA constructs. We construct a number of complex RNA polygons and grids with high yield and quality. Our approach can further expand the RNA design possibilities and enable downstream biomedical applications.