Advanced dECM-based bioprinting strategy for tissue regeneration

The limitations of tissue injury repair, including donor shortages, immune rejection, and rehabilitation challenges, necessitate the development of functional substitute tissues that promote endogenous regeneration and can be produced at scale. This review focuses on the advancements in decellularized extracellular matrix (dECM)-based three-dimensional bioprinting techniques, which combine the structural integrity of dECM with the spatial precision of bioprinting. Utilizing pluripotent stem cells in a three-dimensional microenvironment, organoids derived through these methods demonstrate enhanced cell adhesion, proliferation, and differentiation while maintaining their architectural and functional integrity. Our principal findings highlight that integrating dECM with bioprinting significantly improves tissue engineering models stability and maturation, enabling personalized tissue engineering using patient-derived cells and matched dECM. This integration facilitates innovative applications in disease modeling and drug testing. We conclude with practical recommendations for advancing standardization, scalability, and regulatory translation in the field, emphasizing the critical role of these technologies in improving tissue repair outcomes. • DECM-enhanced 3D bioprinting enables scalable, patient-specific tissue models. • Precise bioink placement with preserved native ECM boosts maturation and function. • Integrated platforms support disease modeling, drug testing, and regenerative translation.