Metabolic analysis of MYB30 that regulates iron deficiency stress in Arabidopsis

Phenotype analysis demonstrated that MYB30 mutant plants were sensitive to iron deficiency, exhibiting reduced root length, lower chlorophyll content, and elevated lipid peroxidation, whereas MYB30 overexpression lines showed enhanced tolerance. Metabolomic analysis of myb30 plant roots by mass spectrometry indicated decreased antioxidant activity and detoxification capacity under iron-deficient conditions. Interestingly, 22 metabolic pathways were altered in the <i>myb30</i> plant under iron deficiency. This metabolic reprogramming likely compromises plant growth. Furthermore, MYB30 reduced reactive oxygen species accumulation under iron deficiency stress by activating related genes and enhancing antioxidant enzyme activity. In summary, metabolite analysis provides detailed molecular insights into plant iron deficiency stress and supports molecular genetic breeding efforts to improve mineral nutrition in crops.