Institut Georges Lopez-2M as a Novel Lung Preservation Solution Attenuates Ischemia-Reperfusion Injury in a Rat Ex Vivo Lung Perfusion Model

<i>Institut</i> <i>Georges Lopez-2M</i> (<i>IGL-2M</i>), a novel preservation solution containing polyethylene glycol (PEG 35kD, 5 g/L), preserves mitochondrial integrity and redox balance in liver grafts. This study assesses <i>IGL-2M</i>'s effect on lung preservation during prolonged cold ischemia. Rat's heart-lung blocks were procured and subjected to 18 h cold ischemia (4 °C). Lungs were flushed and preserved using one of these preservation solutions: <i>OCS</i>, <i>Perfadex Plus</i>, <i>IGL-2M</i> (n = 6/group). Following ischemia, lungs underwent up to 7 h normothermic <i>ex vivo</i> lung perfusion. Edema was quantified by weight gain. Lung physiological parameters were recorded. Perfusate, bronchoalveolar lavage (BAL), and tissue samples were collected. All lungs in <i>IGL-2M</i> group completed 7 h EVLP protocol. Compared to <i>OCS</i>, <i>IGL-2M</i> reduced edema formation (p < 0.01), preserved superior compliance (p < 0.01), and maintained lower pulmonary vascular resistance (p < 0.01). <i>IGL-2M</i> showed lower perfusate concentrations of IL-1β (p < 0.05), IL-6 (p < 0.05), and TNF-α (p = 0.08). In BAL, <i>IGL-2M</i> reduced IL-1β (p < 0.01), IL-6 (p < 0.05), TNF-α (p < 0.01), and CXCL1 (p < 0.01). <i>IGL-2M</i> showed lower release of Syndecan-1 (p < 0.05). Compared to <i>Perfadex Plus</i>, <i>IGL-2M</i> was not inferior, with reduced expression of TNF-α in the perfusate (p < 0.05). <i>IGL-2M</i> effectively prevents edema development like <i>Perfadex Plus</i>. <i>IGL-2M</i> results in decreased inflammation and a stronger endothelial lining, making it a promising solution for lung preservation.