Validation of the Primerdesign Quantitative Allele Specific Amplification Kit for the Detection of <scp>JAK2V617F</scp> Mutation
The PrimerDesign Quasa kit for the detection of JAK2V617F is a sensitive and reliable assay that yields reproducible qualitative results. The kit is commercially available and has, in addition, the potential for quantitative analysis when samples are analysed in duplicate. This kit assay validation may be valuable to laboratories offering or interested in offering JAK2V617F mutational testing.
Authors
- Johan Hendrik Potgieter
- Tshiphiri Netshidzivhani (ORCID: https://orcid.org/0000-0002-0655-3883)
- Deborah Vaz
Institutions
- National Health Laboratory Service (ZA)
- Tshwane University of Technology (ZA)
- University of Pretoria (ZA)
Publication Details
- Journal
- Journal of Clinical Laboratory Analysis
- Published
- 2026-02-19
- DOI
- https://doi.org/10.1002/jcla.70180
- Primary Topic
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- University of Pretoria
- National Health Laboratory Service