Genomic and phenotypic analysis of Weissella cibaria LB13201 and LB13206 isolated from Hanwoo (native Korean cattle) with antimicrobial and anti-inflammatory capability

Probiotics are widely used in livestock to improve gut health, enhance productivity, and increase disease resistance. <i>Weissella</i> strains, particularly <i>W. cibaria,</i> have probiotic properties. However, research on the <i>Weissella</i> genus remains limited. This study investigated the genomic, functional, and probiotic characteristics of <i>W. cibaria</i> strains LB13201 and LB13206, which were isolated from the bovine vagina. Whole-genome sequencing revealed that the strains possess circular chromosomes with two (LB13206) or three (LB13201) plasmids, containing 2,253 and 2,311 coding sequences, respectively. Taxonomic classification confirmed its identity as <i>W. cibaria</i>. The analyzed strains exhibited tolerance to acidic environments and bile salts, along with auto- and co-aggregation capabilities, indicating potential for gut colonization and pathogen exclusion. Functional gene analysis identified carbohydrate-active enzymes, such as glycoside hydrolases and glycosyltransferases, essential for carbohydrate metabolism and food fermentation. Additionally, the presence of a Type III polyketide synthase gene and a terpene-precursor cluster suggests potential for bioactive metabolite production. Despite the absence of bacteriocin-encoding genes, <i>W. cibaria</i> LB13201 and LB13206 displayed antimicrobial activity against pathogens. Comprehensive safety assessments confirmed the absence of virulence factors, antibiotic resistance genes, hemolytic activity, or gelatinase production, supporting its safety profile. The strain also exhibited antioxidant and anti-inflammatory properties, suggesting additional health benefits. In conclusion, <i>W. cibaria</i> LB13201 and LB13206 are promising probiotic candidates for potential application in cattle feed additives.