Development and Application of Chemical Biology Tools to Interrogate ADPr Ubiquitination

ADP-ribosyl ubiquitination is a unique form of crosstalk between post-translational modifications, where recently RNF114 has been identified as the first dedicated reader of this dual modification. New findings reveal that RNF114 extends the initial hybrid ADPr-Ub modification with K11-linked poly-Ub chains. This <i>Letter</i> highlights the recent advances made in methods and tools to study this hybrid signal and addresses some key questions that remain.