Engineering Pseudomonas taiwanensis VLB120 for regio- and stereospecific hydroxylation of l-lysine fueled by the Weimberg pathway
We successfully established a whole-cell biocatalyst for the synthesis of hydroxy-L-lysine from L-lysine and D-xylose and demonstrated multigram-scale production with our engineered strain. Our work lays the foundation for whole-cell bioprocesses utilizing Fe<sup>2+</sup>/α-ketoglutarate-dependent oxygenases fueled by the Weimberg pathway.
Authors
- Philipp Nerke
- Stephan Lütz (ORCID: https://orcid.org/0000-0001-8534-0554)
- Julian Handke
- Georg Hubmann
Institutions
- TU Dortmund University (DE)
Publication Details
- Journal
- Microbial Cell Factories
- Published
- 2026-02-19
- DOI
- https://doi.org/10.1186/s12934-026-02931-0
- Primary Topic
- Metal-Catalyzed Oxygenation Mechanisms
- Type
- article
- Field-Weighted Citation Impact
- 0.00