Protocol for rapid allelic discrimination qPCR genotyping of the <i>Winnie</i> mouse model

Allelic discrimination qPCR protocol for genotyping the <i>Muc2</i> p.Cys52Tyr mutation using dual-labeled hydrolysis probes Enables rapid discrimination of wild-type, heterozygous, and mutant alleles in a single reactionCompatible with standard real-time PCR instruments and requires no post-PCR processingSupports high-throughput genotyping from crude DNA with minimal hands-on time.

Authors

Institutions

Publication Details

Journal
bioRxiv (Cold Spring Harbor Laboratory)
Published
2026-02-18
DOI
https://doi.org/10.64898/2026.02.17.704640
Primary Topic
Inflammatory Bowel Disease
Type
article
Field-Weighted Citation Impact
0.00

Funders

Controls
|||
ALL TIME
JAN
FEB
MAR
APR
MAY
JUN
JUL
AUG
SEP
OCT
article

Protocol for rapid allelic discrimination qPCR genotyping of the Winnie mouse model

Behzad Mansoori
bioRxiv (Cold Spring Harbor Laboratory)
Inflammatory Bowel Disease
article

Protocol for rapid allelic discrimination qPCR genotyping of the Winnie mouse model

Behzad Mansoori
article en

Abstract

Allelic discrimination qPCR protocol for genotyping the <i>Muc2</i> p.Cys52Tyr mutation using dual-labeled hydrolysis probes Enables rapid discrimination of wild-type, heterozygous, and mutant alleles in a single reactionCompatible with standard real-time PCR instruments and requires no post-PCR processingSupports high-throughput genotyping from crude DNA with minimal hands-on time.

bioRxiv (Cold Spring Harbor Laboratory)
The Wistar Institute (US)
National Institutes of Health
Reduced inequalities
Openalex Percentile: Top 48%
Inflammatory Bowel Disease
DOIOpen Access PDFOpenAlex
AI Navigator

Ask Laika to Summarize, Analyze, and Connect papers live on the map.

Summarize Papers & Methodologies

Extract key findings, datasets, and comparative methods across publications.

Benchmark Rankings & Visual Analytics

Rank top research institutions, authors, funders, topics, and journals by Field-Weighted Citation Impact (FWCI) and paper volume with instant charts.

Connect Distant Disciplines

Bridge topological clusters on the map to find hidden collaborative intersections.

Protocol for rapid allelic discrimination qPCR genotyping of the <i>Winnie</i> mouse model — Behzad Mansoori · bioRxiv (Cold Spring Harbor Laboratory) (2026) | TGRS Research Map | TGRS