Age-related Delays in Osteochondral Remodeling of Fracture Healing Illustrated by Mass Spectrometry Imaging
Using spatially-resolved proteomics via mass spectrometry imaging on fracture callus tissues from young and aged mice, we observed delayed healing in aged animals based on the composition of the extracellular matrices. Higher levels of bone specific collagens were detected in young animals, whereas cartilage specific collagens were detected in aged animals at higher levels. Further, detection of novel, non-canonical callus proteins revealed critical transitional steps that are delayed in aged-callus tissues, and these may also contribute to the delayed healing aged animals.
Authors
- Charles A. Schurman (ORCID: https://orcid.org/0000-0002-5739-8481)
- Peggi M. Angel (ORCID: https://orcid.org/0000-0002-4436-555X)
- Nannan Tao
- Harrison B. Taylor
- Diane Hu
- Ralph Marcucio
- Birgit Schilling
- Theodore Miclau
- William Chandler
Institutions
- San Francisco General Hospital (US)
- Buck Institute for Research on Aging (US)
- Medical University of South Carolina (US)
- Bruker (United States) (US)
Publication Details
- Journal
- bioRxiv (Cold Spring Harbor Laboratory)
- Published
- 2026-02-18
- DOI
- https://doi.org/10.64898/2026.02.13.705828
- Primary Topic
- Bone fractures and treatments
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Institutes of Health
- National Institute on Aging
- National Cancer Institute
- National Institute of Arthritis and Musculoskeletal and Skin Diseases