Seroprevalence and optimized quantitative PCR detection of Entamoeba histolytica using self-collected rectal swabs among men who have sex with men in southern Taiwan: a cross-sectional study

The present study investigated E. histolytica infection among non-HIV MSM in Taiwan using ELISA IgG and novel molecular diagnostic methods. The seroprevalence of 7.9% suggests ongoing transmission in this population. The optimized self-collected rectal swab protocol combined with the novel PCR assay provides a practical surveillance tool. Further investigation and targeted prevention strategies are warranted.