Application of a small molecule calcium influx inducer as a vaccine adjuvant: enhancing Th2-biased immune responses
<b>2G272</b> activated mBMDCs associated with EV release and a store-operated calcium entry response. Enhanced cognate T cell proliferation was mediated either through direct engagement with compound-stimulated mBMDCs or indirectly via immunostimulatory extracellular vesicles released by <b>2G272</b>-activated mBMDCs. <b>2G272</b> elicited minimal systemic cytokine and chemokine release, demonstrating a promising safety profile. When used as an adjuvant in a murine vaccination model, <b>2G272</b> enhanced the IgG1 response with an associated T helper 2 cytokine profile. Hence this compound shows promise as an adjuvant if a Th2 response is beneficial or in combination with other agents to provide a balanced immune response in vaccines.
Authors
- Tyler H.T. Brown
- Fernando Gil (ORCID: https://orcid.org/0000-0001-6691-5340)
- Nikunj M. Shukla (ORCID: https://orcid.org/0000-0002-5150-7827)
- Y Sako (ORCID: https://orcid.org/0000-0002-9279-1449)
- Jasmine Jin (ORCID: https://orcid.org/0009-0001-2037-0936)
- Tomoko Hayashi (ORCID: https://orcid.org/0000-0002-9589-8348)
- Arthur W. H. Chan (ORCID: https://orcid.org/0000-0001-7392-4237)
- Renna Cozza
- Paola Anguiano Quiroz
- Shiyin Yao
- Yumi Yokoyama
- Ian Mclaughlin
- Dennis A. Carson
- Maripat Corr
Institutions
- University of California San Diego (US)
Publication Details
- Journal
- Frontiers in Immunology
- Published
- 2026-02-17
- DOI
- https://doi.org/10.3389/fimmu.2026.1704416
- Primary Topic
- Immunotherapy and Immune Responses
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- U.S. Department of Health and Human Services
- Frederick National Laboratory for Cancer Research
- National Institutes of Health
- University of California, San Diego
- National Institute of Allergy and Infectious Diseases