An automated morphometric approach to evaluate distal lung patterning in mouse models of bronchopulmonary dysplasia

We report a semi-automated method of quantitating the characteristics of large and small terminal respiratory airways. This tool expedites analysis and removes operator bias relative to existing methods. We also demonstrate that LM changes in an acute model of hyperoxia-induced BPD result from both alveolar simplification and inadequate primary septation at the level of the alveolar ducts.