Chromosome-level genome assembly of the Leopard Wrasse Macropharyngodon Meleagris

Macropharyngodon meleagris is a coral reef-dwelling, benthic predatory fish renowned for its striking coloration and distinctive body patterns. It exhibits pronounced sexual dimorphism and is characterized by the presence of prominent canine-like teeth. Here, we employed PacBio HiFi sequencing combined with Hi-C assembly technology to generate a high-quality, chromosome-level genome assembly of M. meleagris. The final assembly spans 666 Mb across 24 chromosomes, with high contiguity (a contig N50 of 20.57 Mb and a scaffold N50 of 29.79 Mb). Approximately 27.63% of the genome is composed of repetitive elements. A total of 21,940 protein-coding genes were predicted, with 98.76% successfully assigned functional annotations. The assembled genome exhibits high completeness (98.7% BUSCO completeness) and accuracy (98.05% for WGS short reads, 99.86% for HiFi long reads and 92.64% for RNAseq reads).

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Journal
Scientific Data
Published
2026-02-17
DOI
https://doi.org/10.1038/s41597-026-06817-z
Primary Topic
Genomics and Phylogenetic Studies
Type
article
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article

Chromosome-level genome assembly of the Leopard Wrasse Macropharyngodon Meleagris

Chang Li, Meng Qu, Haiyan Yu, Dazhi Wang et al.
Scientific Data
Genomics and Phylogenetic Studies
article

Chromosome-level genome assembly of the Leopard Wrasse Macropharyngodon Meleagris

Chang Li, Meng Qu, Haiyan Yu, Dazhi Wang, Qiang Lin
article en

Abstract

Macropharyngodon meleagris is a coral reef-dwelling, benthic predatory fish renowned for its striking coloration and distinctive body patterns. It exhibits pronounced sexual dimorphism and is characterized by the presence of prominent canine-like teeth. Here, we employed PacBio HiFi sequencing combined with Hi-C assembly technology to generate a high-quality, chromosome-level genome assembly of M. meleagris. The final assembly spans 666 Mb across 24 chromosomes, with high contiguity (a contig N50 of 20.57 Mb and a scaffold N50 of 29.79 Mb). Approximately 27.63% of the genome is composed of repetitive elements. A total of 21,940 protein-coding genes were predicted, with 98.76% successfully assigned functional annotations. The assembled genome exhibits high completeness (98.7% BUSCO completeness) and accuracy (98.05% for WGS short reads, 99.86% for HiFi long reads and 92.64% for RNAseq reads).

Scientific DataVol. 13(1)
Xiamen University (CN), South China Sea Institute Of Oceanology (CN), Sanya University (CN), University of Chinese Academy of Sciences (CN)
Life below water
Openalex Percentile: Top 53%
Genomics and Phylogenetic Studies
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Chromosome-level genome assembly of the Leopard Wrasse Macropharyngodon Meleagris — Chang Li, Meng Qu, et al. · Scientific Data (2026) | TGRS Research Map | TGRS