Oncorhynchus milt DNA supplementation combined with voluntary exercise alters exercise-related energy substrate utilization of skeletal muscle in mice

Lipid utilization is essential for continued exercise. Wheel running is a well-established voluntary exercise, and FAT/CD-36, a fatty acid transporter, is more active during exercise to initiate lipid metabolism in the muscle. Deoxyribonucleic acid (DNA) supplementation has been suggested to be involved in the transmission of genetic information, and enhancing the fatty acid oxidation. Therefore, the combined wheel running with OmDNA intake has the potential to enhance endurance capacity by improving lipid utilization. Mice were randomly assigned to four groups: sedentary control (CON), sedentary along with Oncorhynchus milt DNA (OmDNA) supplementation (DNA), exercise (EX), and exercise along with OmDNA supplementation (EX+DNA). The time to exhaustion during the running test showed no statistically significant difference between the EX+DNA and the EX groups. The blood lactate levels after the running test in the EX+DNA group were significantly lower than those in the CON, DNA, and EX groups. The protein levels of FAT/CD-36 were significantly increased in the Ex+DNA group compared with the EX group. Conclusions : The combination of wheel running and nucleic acid supplementation likely reflects a metabolic response that enhances lipid utilization and reduces glucose consumption by activating fatty acid oxidation via increased expression of the FoxO/FAT/CD-36 pathways in skeletal muscle in the present study.