Indirect estimation of serum enzymes reference intervals in adults using the reflimR and refineR algorithms

ReflimR provides a practical approach for indirect RIs estimation from routine data. Its performance was comparable to refineR and parametric methods, supporting its use for verifying or updating local RIs, especially where population-specific RIs are unavailable. To our knowledge, this is the first study to apply reflimR to the Turkish population and directly compare its performance with refineR and IFUs.