The Strand Cycling Amplification Nanosensor Platform: A Bioinformatic-Guided Biosensor for Single-Nucleotide-Specific Detection of <i> <i>Pseudomonas aeruginosa</i> </i>

<i><i>Pseudomonas aeruginosa</i></i> is a high-priority antibiotic-resistant pathogen that requires rapid and specific diagnostic methods. We present an electrochemical biosensor platform named SCAN (Strand Cycling Amplification Nanosensor) for the highly accurate and efficient detection of <i><i>P. aeruginosa</i></i>. By combining bioinformatic screening of species-specific 16S rRNA fragments and the high-fidelity DNA recognition capability of the OMEGA-IsrB nuclease, the SCAN platform integrates enzymatic signal amplification with host-guest chemistry for electrochemical readout. This method achieves exceptional sensitivity, detecting down to 26 CFU/mL of bacterial genomic DNA within 150 min, and exhibits no cross-reactivity with closely related species, fungi, or human cells. When applied to clinical sputum samples, the SCAN platform demonstrated excellent agreement with standard diagnostic methods. With its rapid, culture-free, and precise detection capabilities, this technology offers a promising tool for clinical diagnostics and infection management.