Development of a qPCR assay for Fasciola spp. identification and a deep amplicon sequencing method for differentiation of fluke species in UK livestock
This study developed a qPCR assay for Fasciola spp. detection and validated a deep amplicon sequencing for fluke species differentiation. These approaches are able to identify fluke species in excreta from infected ruminants and provide additional valuable tools for enhancing fasciolosis surveillance and control.
Authors
- Sai Fingerhood (ORCID: https://orcid.org/0000-0003-1588-0672)
- Q ALI (ORCID: https://orcid.org/0009-0000-7619-5033)
- Martha Betson (ORCID: https://orcid.org/0000-0002-4220-3290)
- Moneeb Ashraf (ORCID: https://orcid.org/0000-0002-5769-9524)
- Eric R. Morgan (ORCID: https://orcid.org/0000-0002-5999-7728)
- Iñaki Deza-Cruz (ORCID: https://orcid.org/0000-0002-3754-1084)
- Nick Selemetas
- Umer Chaudhry
- Kezia Kozel (ORCID: https://orcid.org/0009-0009-3448-6989)
- Mark W. Robinson
- Muhammad Abbas
- Isah Ibrahim
- Olukayode Daramola
Institutions
- Queen's University Belfast (GB)
- Ahmadu Bello University (NG)
- Roslin Institute (GB)
- Long Island University (US)
- University of Nottingham (GB)
- University of Lancashire (GB)
- Riphah International University (PK)
- Ross University School of Veterinary Medicine (KN)
- University of Surrey (GB)
- University of Veterinary and Animal Sciences (PK)
Publication Details
- Journal
- PLoS neglected tropical diseases
- Published
- 2026-02-17
- DOI
- https://doi.org/10.1371/journal.pntd.0014006
- Primary Topic
- Helminth infection and control
- Type
- article
- Field-Weighted Citation Impact
- 0.00