Optimizing diagnostic thresholds of total vitamin B12 (B12) for identifying cobalamin deficiency in adults with macrocytic anemia
Among individuals investigated for anemia, macrocytosis, a hallmark of B12 depletion, supports that total B12 concentrations ≤257 ng/L measured using the Roche assay likely reflect severe deficiency. Levels between 258 and 341 ng/L may indicate early depletion and warrant confirmation through elevated homocysteine concentrations.
Authors
- Luca Lalli (ORCID: https://orcid.org/0000-0002-4472-628X)
- Martina Tosi (ORCID: https://orcid.org/0000-0003-4859-5288)
- B. Cesana
- Giulia Musso (ORCID: https://orcid.org/0000-0002-7748-773X)
- Martina Montagnana
- Edoardo Campigotto
- Simona Ferraro
- Gianvicenzo Zuccotti
- Michela Pelloso
Institutions
- University of Padua (IT)
- University of Milan (IT)
- Ospedale dei Bambini Vittore Buzzi (IT)
- Istituti di Ricovero e Cura a Carattere Scientifico (IT)
Publication Details
- Journal
- Clinical Chemistry and Laboratory Medicine (CCLM)
- Published
- 2026-02-13
- DOI
- https://doi.org/10.1515/cclm-2025-1722
- Primary Topic
- Folate and B Vitamins Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00