Highly Sensitive Rhodol‐Based Fluorescent Probe for Detecting Hydrogen Peroxide in Cells and Zebrafish
As a critical reactive oxygen species (ROS), hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) plays a significant role in regulating oxidative stress, which closely relates to various human diseases. Therefore, it is of great significance to sensitively detect H<sub>2</sub>O<sub>2</sub> and elucidate its physiological function. Herein, a fluorescent probe was present for detecting H<sub>2</sub>O<sub>2</sub> in living cells and zebrafish. It exhibited highly selective and sensitive responses in the presence of H<sub>2</sub>O<sub>2</sub> with a detection limit of 142.18 nM. The probe was successfully applied to detect endogenous and exogenous H<sub>2</sub>O<sub>2</sub> in living cells and zebrafish. All the results in this work demonstrated that the probe was a powerful tool for detecting H<sub>2</sub>O<sub>2</sub> and exploring the roles of H<sub>2</sub>O<sub>2</sub> in various physiological and pathological processes.
Authors
- Jiaqi Yu (ORCID: https://orcid.org/0000-0001-7412-2746)
- Bingxin Zhu
- Shihua Yu (ORCID: https://orcid.org/0000-0001-9782-5233)
- Xiaodan Zeng
- Mingshuo Ma
- Shanshan Wang
Institutions
- Jilin University of Chemical Technology (CN)
Publication Details
- Journal
- Luminescence
- Published
- 2026-02-01
- DOI
- https://doi.org/10.1002/bio.70449
- Primary Topic
- Molecular Sensors and Ion Detection
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- People's Government of Jilin Province
- Department of Science and Technology of Jilin Province