Generation of two isogenic sickle cell disease induced pluripotent stem cell lines from ovarian fibroblasts
Curative bone marrow transplantation (BMT) therapies for sickle cell disease (SCD) can cause infertility. The Fertility Preservation Program (FPP) in Pittsburgh cryopreserves ovarian tissues for SCD patients prior to BMT in anticipation that these tissues can be used in the future to produce eggs and achieve a pregnancy. Here, we generated and validated two isogenic patient-derived induced pluripotent stem cell (iPSC) lines from ovarian cortex fibroblasts of a 28-year-old SCD patient.
Authors
- Meena Sukhwani (ORCID: https://orcid.org/0000-0001-5551-2298)
- Mitchell R. Harancher (ORCID: https://orcid.org/0000-0002-7928-2367)
- Amanda C. Zielen (ORCID: https://orcid.org/0000-0002-4291-4084)
- Charvi Kanodia (ORCID: https://orcid.org/0000-0002-2187-516X)
- Kyle E. Orwig (ORCID: https://orcid.org/0000-0002-7952-8419)
- Livianna K. Myklebust (ORCID: https://orcid.org/0000-0003-1838-0024)
- Yi Sheng (ORCID: https://orcid.org/0000-0003-4799-7939)
- Carlos A. Castro
Institutions
- University of Pittsburgh (US)
- Magee-Womens Research Institute (US)
- Magee-Womens Hospital (US)
- MRC Centre for Reproductive Health (GB)
- University of Pittsburgh Medical Center (US)
- The Queen's Medical Research Institute (GB)
Publication Details
- Journal
- Stem Cell Research
- Published
- 2026-02-16
- DOI
- https://doi.org/10.1016/j.scr.2026.103936
- Primary Topic
- Pluripotent Stem Cells Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00