Correlations Between Osteocyte Lacuno‐Canalicular Network and Material Characteristics in Healthy and Mechanically Stimulated Murine Tibiae

This article investigates the heterogeneity of bone nanostructure in 26‐week‐old C57BL/6J mice, focusing on tibial mid‐shaft cross‐sections. We aimed to investigate the relationship between calcium content, mineral nanostructure, lacuno‐canalicular network (LCN) organization, and loading history. Using advanced imaging techniques, including electron microscopy, confocal laser scanning microscopy, and synchrotron X‐ray scattering, we compared material properties in loaded versus non‐loaded bones, as well as regions with high and low calcium content. Our results demonstrate a strong correlation between LCN structure and calcium content, indicating that mineral nanostructure is predominantly influenced by LCN organization. Importantly, our findings highlight the impact of bone formation due to mechanical loading, promoting mature mineral crystal structures despite reduced local calcium content. This suggests that systemic calcium supply drives mineralization during adaptive remodeling. Overall, the article underscores the importance of recognizing structural heterogeneity in bone, enhancing our understanding of the mechanisms underlying bone adaptability and quality, and paving the way for future research into therapeutic strategies aimed at improving skeletal health.