Effect of Jasminum sambac on Stress-Induced Anxiety in Mice

Background: Anxiety disorders are prevalent neuropsychiatric conditions that significantly impair quality of life. Although conventional anxiolytic drugs are effective, their use is limited by adverse effects, prompting the search for safer alternatives from medicinal plants. Jasminum sambac has been traditionally used for central nervous system disorders, but its anxiolytic potential lacks scientific validation. Objective: This study aimed to evaluate the anxiolytic activity of the ethanolic leaf extract of Jasminum sambac in experimental animals. Methods: The ethanolic leaf extract of Jasminum sambac was prepared and subjected to preliminary phytochemical screening. Anxiolytic activity was assessed in Swiss albino mice using the Elevated Plus Maze, Light and Dark Box, Open Field Test, and Forced Swim Test. Biochemical parameters including plasma nitrate, inducible nitric oxide synthase, acetylcholinesterase, monoamine oxidase-A and -B, and glutathione reductase were estimated. Diazepam (4 mg/kg) served as the standard drug. Data were analyzed using one-way ANOVA followed by Dunnett’s test. Results: The extract showed significant, dose-dependent anxiolytic effects, with improved behavioral parameters and normalization of biochemical markers, comparable to diazepam at higher doses. Conclusion: The ethanolic leaf extract of Jasminum sambac exhibits notable anxiolytic activity, supporting its traditional use and potential as a natural anxiolytic agent. Keywords: Anxiety disorders; Jasminum sambac; Elevated Plus Maze; Herbal medicine; GABAergic system; Oxidative stress