Uspešno čiščenje DNK iz agaroznih čepkov PFGE za sekvenciranje celotnega genoma

Whole-genome sequencing (WGS) has replaced Pulsed-field gel electrophoresis (PFGE)-based bacterial genotyping as the reference genotyping method. We investigated the suitability of purified genomic DNA extracted from PFGE agarose plugs stored in a laboratory collection for WGS in cases where bacterial isolates are no longer available. Our study has shown that bacterial WGS can be successfully performed on DNA extracted from PFGE agarose plugs.

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Publication Details

Journal
Repository of the University of Ljubljana (University of Ljubljana)
Published
2026-02-12
Primary Topic
Microbial Community Ecology and Physiology
Type
article
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article

Uspešno čiščenje DNK iz agaroznih čepkov PFGE za sekvenciranje celotnega genoma

Tina Triglav, Mateja Pirš, Andraž Celar Šturm, Katja Strašek Smrdel
Repository of the University of Ljubljana (University of Ljubljana)
Microbial Community Ecology and Physiology
article

Uspešno čiščenje DNK iz agaroznih čepkov PFGE za sekvenciranje celotnega genoma

Tina Triglav, Mateja Pirš, Andraž Celar Šturm, Katja Strašek Smrdel
article en

Abstract

Whole-genome sequencing (WGS) has replaced Pulsed-field gel electrophoresis (PFGE)-based bacterial genotyping as the reference genotyping method. We investigated the suitability of purified genomic DNA extracted from PFGE agarose plugs stored in a laboratory collection for WGS in cases where bacterial isolates are no longer available. Our study has shown that bacterial WGS can be successfully performed on DNA extracted from PFGE agarose plugs.

Repository of the University of Ljubljana (University of Ljubljana)
Openalex Percentile: Top 60%
Microbial Community Ecology and Physiology
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Uspešno čiščenje DNK iz agaroznih čepkov PFGE za sekvenciranje celotnega genoma — Tina Triglav, Mateja Pirš, et al. · Repository of the University of Ljubljana (University of Ljubljana) (2026) | TGRS Research Map | TGRS