The complete chloroplast genome sequence of <i>Camellia obtusifolia</i> (Theaceae)

<i>Camellia obtusifolia</i> is a wild relative of the economically important oil crop <i>Camellia oleifera</i>. Here, we sequenced and analyzed the complete chloroplast genome of <i>C. obtusifolia</i> to clarify its phylogenetic position. The cp genome was 156,540 bp in length and exhibited a typical quadripartite structure comprising a LSC (86,254 bp), a SSC (18,400 bp), and two IR (25,943 bp) regions. It contained 134 genes, including 88 protein-coding genes, 37 tRNA genes, and eight rRNA genes, with a GC content of 37.3%. Comparative analysis revealed a 452-bp deletion in the <i>atp</i>H<i>-atp</i>I intergenic region, which served as a distinguishing feature between <i>C. obtusifolia</i> and <i>C. oleifera</i>. Phylogenetic analysis showed that <i>C. obtusifolia</i> clustered into a clade with <i>Camellia sasanqua</i>, <i>Camellia brevistyla</i>, and <i>Camellia meiocarpa</i>, demonstrating the closest relationship with <i>C. sasanqua</i> (bootstrap = 98%), contradicting previous morphological classifications. This study provided essential genomic resources for future phylogenetic and conservation studies in genus <i>Camellia</i>.