Impact of chaotropic salts on separation simplification on liquid chromatographic determination of cisplatin, pheniramine and their related compound

In this work, we present the beneficial roles of chaotropic agents (salts) in the development of fast, simple, and cost-effective reversed-phase high-performance liquid chromatography with a Diode Array detector. Inclusion of the presence of strong chaotropic salts in mobile phase in their liquid chromatography determinations showed significant simplifications and shortening of run time of analysis. The roles of the two strongest chaotropic agents, salts, potassium hexafluorophosphate and sodium perchlorate in two different and independent research cases of two molecules of active pharmaceutical ingredients, cisplatin and pheniramine maleate, each with its specific related compounds and their pharmaceutical dosage forms, confirmed their highly applicable presence and usability in drug analytical method development. Two separate experiments, using different variable sets, were successfully conducted and presented, one with the strongest chaotropic anion -PF₆^- interacting with cisplatin analyte and alkyl-bonded C18 and C8 reversed phase and the second with the ClO₄^- chaotropic anion with pheniramine analyte and phenyl-bonded phase. Proposed methods were linear in the concentration range 5-30 µg/ml with R² > 0.9990 for the determination of pheniramine and 0.0125-0.75 µg/ml with R² > 0.9997 for the determination of cisplatin. All validation parameters met the acceptability criteria for linearity, accuracy and precision. The summary impacts of the beneficial roles of chaotropic agents express green analysis in the presence of impurities and symmetrical peak for the proposed HPLC methods, cost-efficiency calculated and manifested by run time, organic chemical consumption, equipment duration of exploitation and time labor reduction.