Rapid Detection of Fungi in Nail Specimens Using Multi‐Tube Recombinase Polymerase Amplification Assay

Compared with fungal culture and microscopy, the multi-tube RPA assay demonstrated superior sensitivity while maintaining good specificity, facilitating the identification of fungal pathogens at the species level in nail specimens. Its operational simplicity, enhanced diagnostic performance and reduced processing times make it a promising alternative for the mycological diagnosis of onychomycosis.

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Publication Details

Journal
Mycoses
Published
2026-02-01
DOI
https://doi.org/10.1111/myc.70162
Primary Topic
Nail Diseases and Treatments
Type
article
Field-Weighted Citation Impact
0.00

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article

Rapid Detection of Fungi in Nail Specimens Using Multi‐Tube Recombinase Polymerase Amplification Assay

Rongjie Wu, Songchao Yin, Liyan L. XI, Ling Hu et al.
Mycoses
Nail Diseases and Treatments
article

Rapid Detection of Fungi in Nail Specimens Using Multi‐Tube Recombinase Polymerase Amplification Assay

Rongjie Wu, Songchao Yin, Liyan L. XI, Ling Hu, MeiRong Li, Weian Du, Jing Zhang, Huaiqiu Huang, Zetao Chen
article en

Abstract

Compared with fungal culture and microscopy, the multi-tube RPA assay demonstrated superior sensitivity while maintaining good specificity, facilitating the identification of fungal pathogens at the species level in nail specimens. Its operational simplicity, enhanced diagnostic performance and reduced processing times make it a promising alternative for the mycological diagnosis of onychomycosis.

MycosesVol. 69(2)
Sun Yat-sen University (CN), Sun Yat-sen Memorial Hospital (CN), Third Affiliated Hospital of Sun Yat-sen University (CN)
National Natural Science Foundation of China, China Postdoctoral Science Foundation
Openalex Percentile: Top 54%
Nail Diseases and Treatments
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