Establishment of an induced pluripotent stem cell line (cmESF-iPSC-C3) from cynomolgus monkey ear fibroblasts

Induced pluripotent stem cells (iPSCs) derived from cynomolgus monkey (crab-eating macaque) ear fibroblasts offer a minimally invasive and ethically acceptable source of cells for autologous therapies and preclinical research. Owing to close genetic and physiological similarities to humans, these iPSCs represent a relevant and versatile alternative to human-based models. Here, iPSCs exhibited trilineage differentiation and were directly differentiated into neurons in vitro, confirming pluripotency and neuronal potential. Validation tests confirmed chromosomal integrity, transgene clearance, and mycoplasma-free status. These findings highlight the potential of cynomolgus monkey iPSCs in advancing regenerative medicine, neuroscience research, and developing novel therapeutic strategies for neurodegenerative diseases.