A SYNOPSIS OF ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY (UPLC)

UPLC is refers to Ultra Performance Liquid Chromatography, which improves in three area that are speed, resolution and sensitivity. It is works on the principle of van deemter equation. It is used for fine particles (less than 2 μm), which can reduce the length of column, save time and reduce solvent consumption. UPLC plays a crucial role in separation and quantification of API, Excipients and Impurities in pharmaceutical product .UPLC is used for analysis of biological sample fluids, dosage form and determines the accurate concentration of plant extracts and Pharmacokinetic parameters. It is reducing the analysis time up to 9 times as compared to other chromatographic system. It has been under high pressure (100mpa).In 21st century uplc is suitable for the drug development within short period of time.