TCEP‐Mediated Protein Hydrolysis: Highly Selective Cleavage C‐Terminal to Aspartic Acid

The broader analytical implications of this finding are that many previously unknown origin artifacts can now be properly attributed to a source, particularly in areas such as proteomic sample preparation, redox chemistries studies, and similar. This also expands our knowledge of TCEP chemistry beyond simply a disulfide bond reductor, offering both methodological opportunities for peptide mapping and important cautionary implications for biochemical sample treatment.