Computational Optimization of CRISPR-Cas13a sgRNAs Targeting the SARS-CoV-2 Spike Gene for SHERLOCK-Based Diagnostics
This study successfully designed an sgRNA with high sensitivity and specificity for rapid SARS-CoV-2 detection using the CRISPR-Cas13a system, informed by genomic analysis of Iranian isolates. The proposed approach provides an efficient framework for the rapid design and deployment of CRISPR-based diagnostic tools applicable to diverse viral pathogens.
Authors
- Mohammad Hossein Sanati (ORCID: https://orcid.org/0000-0002-0292-7673)
- Fatemeh Akbarian (ORCID: https://orcid.org/0000-0002-4009-0827)
- Maryam Ahmadzadeh
- Fatemeh Farrokhi
- Hanieh Motaharirad
Institutions
- Royan Institute (IR)
- Academic Center for Education, Culture and Research (IR)
- Sadra Institute Of Higher Education (IR)
- National Institute of Genetic Engineering and Biotechnology (IR)
Publication Details
- Journal
- Evolutionary Bioinformatics
- Published
- 2026-02-01
- DOI
- https://doi.org/10.1177/11769343251414318
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.00